Scientists Reveal How Proteins Team Up To Repair DNA

Scientists have revealed an important mechanism in the repair of
DNA double-strand breaks, according to new research published today
in eLife. Phys.Org reports: One of the main DNA repair processes is
called homologous recombination (HR). This repairs a severe form of
DNA damage where both strands of DNA are broken. A protein called
Rad51 orchestrates HR, and Rad51 itself is supported by several
‘helper’ proteins. The researchers started by using yeast cells to
study Rad51 and its helper proteins, called Swi5-Sfr1. They
genetically engineered yeast cells so that they lacked either
Module 1 or Module 2 of Swi5-Sfr1 and found that this prevented DNA
repair by HR. This shows that both modules are needed for Rad51 to
switch on HR repair. Next, they purified the Swi5-Sfr1 helper
proteins from cells to identify the precise regions within Module 1
that attach to Rad51. Then, by mutating the protein sequence, they
were able to modify these regions in a way that prevents Swi5-Sfr1
from attaching to Rad51. Surprisingly, they found that although the
mutated helper proteins could not switch on Rad51 in a test tube,
yeast cells with these mutations were still able to repair their
DNA without problems. This led the team to speculate that another
group of helper proteins, which are present in the cell but absent
in the test tube, was rescuing the DNA repair process. Previous
genetic studies have shown that there are two HR sub-pathways in
yeast — one that depends on Swi5-Sfr1 and another that relies on
molecules called Rad51 paralogs. To test whether it was this other
HR pathway that was rescuing DNA repair, the team used yeast that
lacked the Rad51 paralogs. The results were striking: in yeast with
mutant Swi5-Sfr1 and no Rad51 paralogs, the DNA damage was much
more severe. This suggests that the damaging effects of mutations
to the Swi5-Sfr1 helper complex are suppressed by a second group of
helper proteins.


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Source: *FS – All – Science News 2 Net
Scientists Reveal How Proteins Team Up To Repair DNA